Bovine Sperm Fractionation

Ethanolic fractionation of bovine testicular hyaluronidase.

Studies on the fractionation of blood serum by Cohn et al. (1) have demonstrated that under precisely controlled conditions reproducible fractionation of a complex mixture of proteins can be accomplished by the use of ethanol. This paper reports the application of this method to the separation of hyaluronidase from testicular extract. A variety of experimental conditions involving variation of ...

Inhibition of bovine sperm-zona binding by bovine herpesvirus-1.

The purpose of the present study was to identify a potential interference of bovine herpesvirus-1 (BoHV-1) with sperm-oocyte interactions during bovine in vitro fertilization. An inhibition of almost 70% of sperm-zona binding was observed when bovine cumulus-denuded oocytes were inseminated in the presence of 10(7) 50% tissue culture infective dose/ml BoHV-1. The inhibitory effect of BoHV-1 on ...

Imminent Commercialization of Sexed Bovine Sperm

Expression of Syntaxin 2 in Bovine Sperm.

Syntaxins are membrane integrated Q-SNARE proteins known to participate in exocytosis. Vesicle docking involves the binding of two plasma membrane proteins, syntaxin and SNAP-25, to the vesicle membrane protein VAMP to form a stable trimeric core complex; synaptophysin is thought to regulate the formation of this complex. Although the members of Q-SNARE proteins are characterized in somatic cel...

Freezing Bovine Sperm with Egg Yolk

Bull spermatozoa were pellet frozen on dry ice in a TES buffer containing combinations of egg yolk and glycerol to determine which component of an extender afforded cryoprotection to the spermatozoa. Neither buffer nor buffer plus glycerol protected sperm cell motility in the absence of egg yolk, while sperm cells frozen in egg yolk buffer in the absence of glycerol had 24% postthaw motility (P...